TRYPAN BLUESOLUTION FOR MICROSCOPY 0.4% (C005B-058971)

General description

Trypan Blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.

Application

Trypan blue applications include:

• it is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents.
• employed as a collagen stain in a Van Gieson procedure
• To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains.
• used as a fluorescent tracer of cell populations in embryology
• used as a tumor promoter modulating permeability of lysosomal membranes

Trypan Blue solution has also been used in the cytotoxity tests and to determine the vital cells for the analysis of cell division.

Biochem/physiol Actions

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

Physical form

0.4% in 0.81% sodium chloride; 0.06% potassium phosphate, dibasic

Molecular Weight: 960.81. Empirical Formula: C₃₄H₂₄N₆Na₄O₁₄S₄. grade: for microscopy. Quality Level: 200. form: liquid. shelf life: limited shelf life, expiry date on the label. concentration: 0.4%. density: 1.007 . g/mL . at 20 . °C. ε. _max: 1.4 at 603 . nm in methanol. application(s): diagnostic assay manufacturing
hematology
histology. storage temp.: room temp. SMILES string: [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N: Nc2c(O)c3c(N)cc(cc3cc2S([O-])(: O): O)S([O-])(: O): O)-c4ccc(N: Nc5c(O)c6c(N)cc(cc6cc5S([O-])(: O): O)S([O-])(: O): O)c(C)c4. InChI: 1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42. . . . /h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54). . . . /q. 4*+1/p-4. InChI key: GLNADSQYFUSGOU-UHFFFAOYSA-J. Pictograms: GHS08. Signal Word: Danger. Hazard Statements: H350. Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501. Hazard Classifications: Carc. 1B. Storage Class Code: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type ABEK (EN14387) respirator filter.