TRIZMA(R) HYDROCHLORIDE BIOULTRA FOR MOLECULAR BIOLOGY >= 99.0 % AT (C005B-010562)

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma hydrochloride is used as a general reagent for the preparation of all types of Tris buffers.

Other Notes

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Component of extraction buffer e.g. phenol extraction of DNA or RNA; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Molecular Weight: 157.60. Empirical Formula: C₄H₁₁NO₃ · HCl. linearFormula: NH₂C(CH₂OH)₃ · HCl. grade: for molecular biology. Quality Level: 200. product line: BioUltra. Assay: ≥. 99.0% (AT). form: powder or crystals. impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected. ign. residue: ≤. 0.2% (as SO₄). loss: ≤. 0.2% loss on drying, 110 °C. pH: 2.5-4.0 (25 . °C, 4 . M in H₂O). useful pH range: 7.0-9.0. pKa (25 °C): 8.1. mp: 150-152 . °C. solubility: H₂O: 4 . M at . 20 . °C, clear, colorless. anion traces: sulfate (SO₄^2-): ≤. 50 . mg/kg. cation traces: Al: ≤. 5 . mg/kg, As: ≤. 0.1 . mg/kg, Ba: ≤. 5 . mg/kg, Bi: ≤. 5 . mg/kg, Ca: ≤. 10 . mg/kg, Cd: ≤. 5 . mg/kg, Co: ≤. 5 . mg/kg, Cr: ≤. 5 . mg/kg, Cu: ≤. 5 . mg/kg, Fe: ≤. 5 . mg/kg, K: ≤. 50 . mg/kg, Li: ≤. 5 . mg/kg, Mg: ≤. 5 . mg/kg, Mn: ≤. 5 . mg/kg, Mo: ≤. 5 . mg/kg, Na: ≤. 50 . mg/kg, Ni: ≤. 5 . mg/kg, Pb: ≤. 5 . mg/kg, Sr: ≤. 5 . mg/kg, Zn: ≤. 5 . mg/kg. λ: 4 . M in H₂O. UV absorption: λ. : 260 . nm A_max: 0.12, λ. : 280 . nm A_max: 0.08. SMILES string: Cl.NC(CO)(CO)CO. InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8. /h6-8H,1-3,5H2. 1H. InChI key: QKNYBSVHEMOAJP-UHFFFAOYSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves.