TRANSFORMING GROWTH FACTOR-B1 HUMAN

General description

Recombinant, human TGF- β1 is produced in CHO (Chinese Hamster Ovary) cells and purified by standard chromatographic techniques.
Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane.

Specificity

Species specificity: Transforming growth factor-β1 (TGF-β1) is effective on human and mouse cells.
Specific activity/EC₅₀: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed.

Application

Transforming growth factor-β1, human (hTGF-β1) has been used to explore function and underlying mechanism of periostin (PN) during orthodontic periodontal ligament remodeling.

Biochem/physiol Actions

Transforming Growth Factor-β1, human (hTGF-β1), is a multifunctional growth factor affecting many functions in a variety of different cells.

Transforming growth factor-β (TGF-β) family comprises of a group of multifunctional cytokines which are involved in controlling apoptosis, growth and differentiation of cells, and play important role during embryo development.

Quality

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
(Note: 1 EU corresponds to 0.1 ng)

Sequence

Chain Length 2 x 112 AA

Two identical polypeptide chains (112 amino acids) connected by disulfide bridges, identical to natural hTGF-β1.

Unit Definition

EC₅₀ definition: The concentration of hTGF-β1 that is required to mediate half maximal inhibition of DNA synthesis (BrdU incorporation) with Mv1-Lu cells.

Preparation Note

Working concentration: 0.01 - 10 ng/ml
Recombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.
Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.
Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Other Notes

For life science research only. Not for use in diagnostic procedures.

biological source: human. Quality Level: 100. recombinant: expressed in CHO cells. Assay: >. 95% (SDS-PAGE). form: solution. potency: <. 0.1 . ng/mL EC₅₀. mol wt: 25,000 . Da. packaging: pkg of 1 . mL (1 μ. g). manufacturer/tradename: Roche. storage condition: avoid repeated freeze/thaw cycles. impurities: <. 0.1 . EU/μ. gtested (LAL test). UniProt accession no.: P01137. storage temp.: −. 20°C. Gene Information: human ... TGFB1(7040). Storage Class Code: 12 - Non Combustible Liquids. WGK: nwg. Flash Point(F): does not flash. Flash Point(C): does not flash.