TMB INSOLUBLE

General description

Insoluble TMB substrate supplied as a single component system. TMB is the most sensitive chromogenic substrate for detection of horseradish peroxidase (HRP)-labeled probes. In the presence of HRP and hydrogen peroxide (H₂O₂), TMB is oxidized first to a blue, cation free radical; upon further reaction with HRP/H₂O₂, or addition of acid, the radical is converted to a yellow diimine terminal oxidation product. TMB is used routinely in ELISA procedures, whereby its soluble oxidation products are measured spectrophotometrically. For TMB to be worthwhile as a blotting reagent, enhancers such as dextran sulfate or other sugar polymers are usually added.This TMB reagent is a safe, single component system that has been developed for use in blotting procedures by a proprietary methodology that eliminates the use of sugar polymers. The reagent produces a stable, insoluble aquamarine precipitate at the reaction site with little or no background. It can significantly increase the detection limits of assays on a variety of membranes. Ideal for many immunoblotting procedures and can significantly increase detection limits of assays on a variety of membranes

Peroxidase substrate that produces a stable, insoluble, aquamarine blue precipitate at reaction site with little or no background.

Warning

Toxicity: Carcinogenic / Teratogenic (D)

Physical form

1.19 mM H₂O₂ in a proprietary solvent, 1.13 mM TMB, pH 4.9 ± 0.03.

Reconstitution

Discard if the solution turns blue or turbid. The reagent is stable at room temperature; refrigeration is recommended for long-term storage. Warm to assay temperature before use.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

form: liquid. Quality Level: 100. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, protect from light. color: clearlight brown. shipped in: ambient. storage temp.: 2-8°C. InChI: 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3. InChI key: UAIUNKRWKOVEES-UHFFFAOYSA-N. Pictograms: GHS07,GHS08. Signal Word: Warning. Hazard Statements: H302 - H351 - H413. Precautionary Statements: P202 - P264 - P273 - P280 - P301 + P312 - P308 + P313. Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.