TETRAZOLE SOLUTION FOR DNA SYNTHESIS 0.45 M IN ACETONITR. (C005B-029661)

General description

The product is ~0.45M solution of tetrazole in acetonitrile. Tetrazole ring plays significant role in various synthetic and industrial processes.

Application

Tetrazole solution is useful for DNA synthesis. It may be employed as a catalyst for the in situ synthesis of deoxyribonucleoside phosphoramidites. It may be used in the preparation of following anionic nucleotide-lipids:

• thymidine 3′-(1,2-dilauroyl-sn-glycero-3-phosphate) , diC₁₂-3′-dT
• thymidine 3′-(1,2-dimyristoyl-sn-glycero-3-phosphate) diC₁₄- 3′-dT
• thymidine-3′-(1,2-dipalmitoyl-sn-glycero-3-phosphate) diC₁₆- 3′-dT

Caution

Saturated solution at room temperature; storage below room temperature causes precipitation.

Other Notes

Catalyst used in the phosphite triester method of oligonucleotide synthesis for the coupling of (dialkylamino)phosphines

Molecular Weight: 70.05. Empirical Formula: CH₂N₄. form: liquid. Quality Level: 300. quality: filtered through a 1 μ. m filter. concentration: ~0.45 . M in acetonitrile. technique(s): DNA synthesis: suitable. impurities: ≤. 0.003% water. bp: 84 . °C (lit.). mp: 156-158 . °C (lit.). density: 0.798 . g/mL . at 20 . °C. SMILES string: c1nnn[nH]1. InChI: 1S/CH2N4/c1-2-4-5-3-1/h1H,(H,2,3,4,5). InChI key: KJUGUADJHNHALS-UHFFFAOYSA-N. Pictograms: GHS02,GHS07. Signal Word: Danger. Hazard Statements: H225 - H302 + H312 + H332 - H319. Precautionary Statements: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2. Storage Class Code: 3 - Flammable liquids. WGK: WGK 3. Flash Point(F): 41.0 °F - closed cup. Flash Point(C): 5 °C - closed cup. Personal Protective Equipment: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter.