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sv3t3b mouse sim embryo fibroblast (c15-1310-198)

SV3T3B MOUSE SIM EMBRYO FIBROBLAST (C005B-234369)

Catalog No.
C005B-234369
Manufacturer No.
85011409-1VL
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
VI

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Cell Line Origin Mouse SIM embryo fibroblast Cell Line Description A 3T3 cell line doubly transformed by polyoma then SV40 viruses, probably the fore-runner of SV-T2 (Sigma Catalogue number. 90030801).

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Cell Line Origin

Mouse SIM embryo fibroblast

Cell Line Description

A 3T3 cell line doubly transformed by polyoma then SV40 viruses, probably the fore-runner of SV-T2 (Sigma Catalogue number. 90030801). Demonstrates a high degree of contact inhibition.

Application

Contact inhibition, transformation and virus studies: SV40

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source: mouse embryo. growth mode: Adherent. karyotype: Not specified. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.
UPC:
10101510
Condition:
New
HazmatClass:
Yes
MPN:
85011409-1VL
Temperature Control Device:
Yes

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