SULFORHODAMINE B (C005B-034666)

General description

Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination.

Application

Sulforhodamine B has been used as a dye in the MCF-7 (human breast adenocarcinoma cell line) cell proliferation assay.

Molecular Weight: 580.65. Empirical Formula: C₂₇H₂₉N₂NaO₇S₂. form: powder. Quality Level: 100. composition: Dye content, 75% . solubility: methanol: 1 . mg/mL. λ. _max: 554 nm. ε (extinction coefficient): ≥. 85000 at 554-560 . nm in 0.1 M NaOH in methanol at 0.005 . g/L. application(s): diagnostic assay manufacturing
hematology
histology. storage temp.: room temp. SMILES string: [Na+].CCN(CC)c1ccc2c(OC3: CC(\C: CC3: C2c4ccc(cc4S([O-])(: O): O)S([O-])(: O): O): [N+](\CC)CC)c1. InChI: 1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35. /h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35). /q. +1/p-1. InChI key: SXQCTESRRZBPHJ-UHFFFAOYSA-M. Storage Class Code: 11 - Combustible Solids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).