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sucrose assay kit

SUCROSE ASSAY KIT

Catalog No.
C005B-315740
Manufacturer No.
SCA20-1KT
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
KT

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General description Sucrose is a disaccharide, naturally occurring carbohydrate found in plants and a major product during photosynthesis. Application This kit is for the quantitative, enzymatic determination of sucrose in food and other

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General description

Sucrose is a disaccharide, naturally occurring carbohydrate found in plants and a major product during photosynthesis.

Application

This kit is for the quantitative, enzymatic determination of sucrose in food and other materials. Sucrose is hydrolyzed to glucose and fructose by invertase. Glucose and fructose are phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to sucrose concentration.

UPC:
41116134
Condition:
New
HazmatClass:
No
MPN:
SCA20-1KT

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