STREPTAVIDIN-POD

General description

Streptavidin is covalently coupled to horseradish peroxidase. The conjugate is used for detection of biotin-labelled substances.

Application

Streptavidin-POD has been used for:

• ELISA
• In situ hybridization
• Western blot

It is also used for immunohistochemistry.

Unit Definition

One unit Streptavidin-POD, corresponding to one unit peroxidase, will oxidize 1 μmol of guaiacol tetramer in one minute at +25 °C and pH 7.0.

Unit Conversion: 1 U (+25 °C; guaiacol as substrate; pH7.0) corresponds to 1.24 U (+25 °C; 1.7 mM ABTS as substrate; pH= 6.0) or to 4.0 U (+25 °C; 8.7 mM ABTS as substrate; pH = 5.0).

Physical form

Liquid, stabilized

Preparation Note

Working concentration: 5 - 500 mU/ml (i.e., stock solution can be diluted).Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:

• ELISA: 50 to 200 mU/ml
• Immunohistocytochemistry: 250 to 1000 mU/ml
• Western blot: 200 to 500 mU/ml

Other Notes

For life science research only. Not for use in diagnostic procedures.

form: liquid. Quality Level: 100. packaging: pkg of 1 . mL. manufacturer/tradename: Roche. storage temp.: -10 to -25°C. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H317. Precautionary Statements: P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501. Hazard Classifications: Skin Sens. 1. Storage Class Code: 11 - Combustible Solids. WGK: WGK 2. Flash Point(F): does not flash. Flash Point(C): does not flash.