Starch Agar, 2.5 kg (C003B-239118)

Starch Agar was formulated by Vedder (1) for the cultivation of Neisseria . It is recommended for the detection of starch hydrolysing microorganisms from foods (2) and clinical samples (3). Present formulation is accepted by BIS for detection of starch hydrolysis by Bacillus cereus (4). Peptic digest of animal tissue and meat extract provide nitrogenous compounds, carbon, sulphur, trace elements etc. to the microorganisms. Flood the surface of 24 - 48 hour old culture on Starch Agar with Grams Iodine (S013). Starch hydrolysis is seen as a colorless zone surrounding the colonies. A blue or purple zone indicates that starch is not hydrolyzed. Storage and Shelf-life: Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. Reference: 1. Vedder, 1915, J. Infect. Dis., 16:385. 2. Harrigan W. and McCance M., 1976, Laboratory Methods in Food and Dairy Microbiology, Academic Press Inc. (London) Ltd. 3. Lennette and others (Eds.), 1985, Manual of Clinical Microbiology, 4th ed., ASM,Washington, D.C. 4. Bureau of Indian Standards, IS : 5887 (Part IV) 1976.