SPERMINE TETRAHYDROCHLORIDE BIOULTRA FOR MOLECULAR BIOLOGY >= 99.5% (AT) (C005B-030836)

Application

Used to precipitate DNA from low salt aqueous buffers.

Biochem/physiol Actions

Mixed NMDA agonist/antagonist at the polyamine site. Neuroprotective effects have been observed at high concentrations (1 mM), while neurotoxicity is observed at lower concentrations. It enhances agonist effectiveness at the strychnine-insensitive glycine site. Plays a role in cellular proliferation and differentiation; inhibits neuronal nitric oxide synthase (nNOS).

Mixed NMDA agonist/antagonist at the polyamine site. Plays a role in cellular proliferation and differentiation; inhibits neuronal nitric oxide synthase (nNOS).

Other Notes

Spermine precipitation of DNA

Molecular Weight: 348.18. Empirical Formula: C₁₀H₂₆N₄ · 4HCl. grade: for molecular biology. Quality Level: 300. product line: BioUltra. Assay: ≥. 99.5% (AT). form: powder. impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected. pH: 4.5-7.0 (25 . °C, 1 . M in H₂O). mp: 310-311 . °C (dec.) (lit.). solubility: H₂O: 1 . m at . 20 . °C, clear, colorless to almost colorless. anion traces: sulfate (SO₄^2-): ≤. 50 . mg/kg. cation traces: Al: ≤. 5 . mg/kg, As: ≤. 0.1 . mg/kg, Ba: ≤. 5 . mg/kg, Bi: ≤. 5 . mg/kg, Ca: ≤. 10 . mg/kg, Cd: ≤. 5 . mg/kg, Co: ≤. 5 . mg/kg, Cr: ≤. 5 . mg/kg, Cu: ≤. 5 . mg/kg, Fe: ≤. 5 . mg/kg, K: ≤. 50 . mg/kg, Li: ≤. 5 . mg/kg, Mg: ≤. 5 . mg/kg, Mn: ≤. 5 . mg/kg, Mo: ≤. 5 . mg/kg, Na: ≤. 50 . mg/kg, Ni: ≤. 5 . mg/kg, Pb: ≤. 5 . mg/kg, Sr: ≤. 5 . mg/kg, Zn: ≤. 5 . mg/kg. λ: 1 . M in H₂O. UV absorption: λ. : 260 . nm A_max: 0.2, λ. : 280 . nm A_max: 0.15. SMILES string: Cl.Cl.Cl.Cl.NCCCNCCCCNCCCN. InChI: 1S/C10H26N4.4ClH/c11-5-3-9-13-7-1-2-8-14-10-4-6-12. . . . /h13-14H,1-12H2. 4*1H. InChI key: XLDKUDAXZWHPFH-UHFFFAOYSA-N. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H315 - H319. Precautionary Statements: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313. Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2. Storage Class Code: 13 - Non Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves.