SODIUM PHOSPHATE DIBASIC BIOULTRA FOR MOLECULAR BIOLOGY >= 99.5 % T (C005B-077192)

Application

Sodium phosphate dibasic has been used:

• as a component in phosphate buffer
• in the preparation of MgCl₂ hybridization buffer
• as a component in SSPE solution for the preparation of small RNA hybridisation buffer

Molecular Weight: 141.96. Empirical Formula: Na₂HPO₄. linearFormula: Na₂HPO₄. grade: for molecular biology. Quality Level: 200. product line: BioUltra. Assay: ≥. 99.5% (T). form: coarse powder, fine crystals, powder. impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤. 0.001% total nitrogen (N). loss: ≤. 0.1% loss on drying, 110 °C. pH: 8.8-9.4 (25 . °C, 0.5 . M in H₂O). pKa (25 °C): (1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid). solubility: H₂O: 0.5 . M at . 20 . °C, clear, colorless. anion traces: chloride (Cl^-): ≤. 10 . mg/kg, sulfate (SO₄^2-): ≤. 50 . mg/kg. cation traces: Al: ≤. 5 . mg/kg, As: ≤. 0.2 . mg/kg, Ba: ≤. 5 . mg/kg, Bi: ≤. 5 . mg/kg, Ca: ≤. 10 . mg/kg, Cd: ≤. 5 . mg/kg, Co: ≤. 5 . mg/kg, Cr: ≤. 5 . mg/kg, Cu: ≤. 5 . mg/kg, Fe: ≤. 5 . mg/kg, K: ≤. 50 . mg/kg, Li: ≤. 5 . mg/kg, Mg: ≤. 5 . mg/kg, Mn: ≤. 5 . mg/kg, Mo: ≤. 5 . mg/kg, Ni: ≤. 5 . mg/kg, Pb: ≤. 5 . mg/kg, Sr: ≤. 10 . mg/kg, Zn: ≤. 5 . mg/kg. λ: 0.5 . M in H₂O. UV absorption: λ. : 260 . nm A_max: ≤. 0.025, λ. : 280 . nm A_max: ≤. 0.020. SMILES string: [Na+].[Na+].[H]OP([O-])([O-]): O. InChI: 1S/2Na.H3O4P/c. . 1-5(2,3)4/h. . (H3,1,2,3,4)/q2*+1. /p-2. InChI key: BNIILDVGGAEEIG-UHFFFAOYSA-L. Storage Class Code: 13 - Non Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).