SODIUM DODECYL SULFATE SOLUTION BIOULTRA FOR MOLECULAR BIOLOGY 10 % WATER (C005B-019504)

Application

Sodium dodecyl sulfate solution has been used:

• in the preparation of 2x extraction buffer
• as a component in elution buffer for immunoprecipitation
• in SDS–polyacrylamide gel electrophoresis (PAGE) analysis

Biochem/physiol Actions

Sodium dodecyl sulfate (SDS)/lauryl sulfate, is an ionic detergent has the ability to disrupt biological membranes. It can block ribonuclease (RNase) and deoxyribonuclease (DNase) activity. SDS is one of the main constituents of several reagents, that are used to purify nucleic acids. It is also considered as a permeability enhancer in oral formulations.

Other Notes

In large-scale phenol extraction of RNA - promotes the dissociation of protein from nucleic acids when extracting from biological material

Molecular Weight: 288.38. Empirical Formula: C₁₂H₂₅NaO₄S. linearFormula: CH₃(CH₂)₁₁OSO₃Na. grade: for molecular biology. Quality Level: 200. description: anionic. product line: BioUltra. form: liquid. mol wt: 288.38 . g/mol. concentration: 10% in H₂O. technique(s): HPLC: suitable, protein quantification: suitable. impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected. pH: 5.0-6.5. density: 1.01 . g/mL . at 20 . °C. anion traces: chloride (Cl^-): ≤. 50 . mg/kg, phosphate (PO₄^3-): ≤. 1 . mg/kg. cation traces: Al: ≤. 1 . mg/kg, As: ≤. 0.1 . mg/kg, Ba: ≤. 1 . mg/kg, Bi: ≤. 1 . mg/kg, Ca: ≤. 5 . mg/kg, Cd: ≤. 1 . mg/kg, Co: ≤. 1 . mg/kg, Cr: ≤. 1 . mg/kg, Cu: ≤. 1 . mg/kg, Fe: ≤. 1 . mg/kg, K: ≤. 20 . mg/kg, Li: ≤. 1 . mg/kg, Mg: ≤. 1 . mg/kg, Mn: ≤. 1 . mg/kg, Mo: ≤. 1 . mg/kg, Ni: ≤. 1 . mg/kg, Pb: ≤. 1 . mg/kg, Sr: ≤. 1 . mg/kg, Zn: ≤. 1 . mg/kg. λ: neat. UV absorption: λ. : 260 . nm A_max: 0.3, λ. : 280 . nm A_max: 0.2. SMILES string: [Na+].CCCCCCCCCCCCOS([O-])(: O): O. InChI: 1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15. /h2-12H2,1H3,(H,13,14,15). /q. +1/p-1. InChI key: DBMJMQXJHONAFJ-UHFFFAOYSA-M. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H315 - H319. Precautionary Statements: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313. Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter.