SODIUM ACETATE SOLUTION BIOULTRA FOR MOLECULAR BIOLOGY ~ 3M WATER

Application

Sodium acetate solution has been used:

• in DNA extraction
• in reverse crosslinking and DNA purification
• to precipitate Gata6 (GATA binding protein 6) cDNA as a part of high-purity plasmid preparation

Biochem/physiol Actions

Sodium acetate serves as a fixed base. It is useful to rectify acidosis among acidotic patients. Orginally, sodium acetate is used as dialysate for hemodialysis, but is known to cause cardiovascular instability.

Other Notes

Basic method of DNA precipitation with ethanol

Molecular Weight: 82.03. Empirical Formula: C₂H₃NaO₂. grade: for molecular biology. Quality Level: 200. product line: BioUltra. form: liquid. concentration: ~3 . M in H₂O. technique(s): precipitation assay: suitable. impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected. pH: 7.0-8.0 (25 . °C, 3 . M in H₂O). anion traces: chloride (Cl^-): ≤. 10 . mg/kg, phosphate (PO₄^3-): ≤. 5 . mg/kg, sulfate (SO₄^2-): ≤. 20 . mg/kg. cation traces: Al: ≤. 1 . mg/kg, As: ≤. 0.1 . mg/kg, Ba: ≤. 1 . mg/kg, Bi: ≤. 1 . mg/kg, Ca: ≤. 5 . mg/kg, Cd: ≤. 1 . mg/kg, Co: ≤. 1 . mg/kg, Cr: ≤. 1 . mg/kg, Cu: ≤. 1 . mg/kg, Fe: ≤. 1 . mg/kg, K: ≤. 50 . mg/kg, Li: ≤. 1 . mg/kg, Mg: ≤. 1 . mg/kg, Mn: ≤. 1 . mg/kg, Mo: ≤. 1 . mg/kg, Ni: ≤. 1 . mg/kg, Pb: ≤. 1 . mg/kg, Sr: ≤. 1 . mg/kg, Zn: ≤. 1 . mg/kg. λ: 3 . M in H₂O. UV absorption: λ. : 260 . nm A_max: ≤. 0.030, λ. : 280 . nm A_max: ≤. 0.024. SMILES string: [Na+].CC([O-]): O. InChI: 1S/C2H4O2.Na/c1-2(3)4. /h1H3,(H,3,4). /q. +1/p-1. InChI key: VMHLLURERBWHNL-UHFFFAOYSA-M. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves.