ERENNA(R) CAPTURE REAGENT LABELING KIT

General description

Take advantage of SMC^® proprietary technology while developing your own application-specific immunoassays. This kit provides everything you need to build your own high performing SMC^® immunoassay. It includes reagents and consumables to label up to 1mg of antibody to SMC^® beads.

The SMC^® immunoassay uses a quantitative fluorescent sandwich immunoassay technique to measure an analyte in a matrix. This kit is intended for the labeling of 0.3-1.0 mg of a capture antibody (Ab) and conjugation to magnetic microparticle (MP) beads. The labeled capture antibody is used in conjunction with a sandwich immunoassay to be developed for the SMC immunoassay system. This kit should be used in conjunction with 03-0076-02 SMC^® detection labeling kit and 03-0078-00 SMC^® bead-based immunoassay development kit.

Application

SMC^® Capture Antibody Labeling Kit has been used to conjugate magnetic particles (MPs):

• to MW1 polyglutamine antibodies for Erenna Singulex single-molecule counting (SMC) immunoassay
• to Covance 4B12 antibody for Singulex assays
• to mouse 5C9-IgG2a for Singulex assay

Components

Capture label
1X Buffer 1
1X Buffer 2
10X Buffer 3
Filter Tube Ultrafree (two)
Ultra-4 30K filters (two)
10X Wash Buffer
Coated bead buffer
Uncoated Beads (10 mg/mL)

Other Notes

Develop your own high-performing detection antibodies and move beyond the traditional ELISA with SMC^® technology. Antibodies used are to be in a carrier- and label-free buffer. If labeling proteins other than antibodies of a 150 kD approximate molecular weight, contact MilliporeSigma technical support. For use in single molecule counting systems.

Legal Information

SMC is a registered trademark of Singulex

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial