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sepharose(r) 4b (c15-1291-081)

SEPHAROSE 4B (C005B-088695)

Catalog No.
C005B-088695
Manufacturer No.
4B200-500ML
Manufacturer Name
Sigma-Aldrich
Quantity
500
Unit of Measure
ML

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Sepharose ™ 4B is an agarose bead and a polymer of Gal ⓵,4 [3,6]-anhydro- L -galactose. Sepharose aids in fractionating larger molecules and is devoid of charged saccharides.

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General description

Sepharose™ 4B is an agarose bead and a polymer of Gal β1,4 [3,6]-anhydro-L-galactose. Sepharose aids in fractionating larger molecules and is devoid of charged saccharides. Presence of 3,6-anhydro-L-galactose resists biological degradation. Hydrogen bonds stabilize the sepharose gel structure. Sepharose is widely used in affinity chromatography by cross linking various ligands on the matrix.

Application

Sepharose™ 4B has been used:

  • to conjugate scorpion venom to be used in affinity chromatography
  • for clearing of protein extracts prior to immunoprecipitation
  • for gel purification and desalting of phospholipid vesicles
  • for adsorption of immunoglobulin G (IgG)

Legal Information

Sepharose is a trademark of Cytiva

biological source: algae (marine). Quality Level: 200. form: suspension. technique(s): affinity chromatography: suitable. bead diameter: 45-165 . μ. m. pore size: 30,000-50,000 . Da fractionation range (Dextrans), 60,000-20,000,000 . Da fractionation range (Globular proteins). storage temp.: 2-8°C. InChI: 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1. InChI key: MJQHZNBUODTQTK-WKGBVCLCSA-N. Pictograms: GHS02. Signal Word: Warning. Hazard Statements: H226. Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P243. Hazard Classifications: Flam. Liq. 3. Storage Class Code: 3 - Flammable liquids. WGK: WGK 1. Flash Point(F): 100.4 - 109.4 °F - closed cup. Flash Point(C): 38 - 43 °C - closed cup.
UPC:
12141738
Condition:
New
HazmatClass:
Yes
MPN:
4B200-500ML
CAS:
9012-36-6

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