R-PROTEIN A SEPHAROSE FAST FLOW 5 ML (C005B-183356)

General description

rProtein A Sepharose^™ 4 Fast Flow is a well established antibody affinity medium, designed for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.

rProtein A Sepharose^™ 4 Fast Flow is based on the well established Sepharose^™ Fast Flow platform and is used in many approved monoclonal antibody (MAb) processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix. The recombinant protein A is produced in E. coli and has been specially engineered to favour an oriented coupling giving a matrix with enhanced binding capacity. The epoxy based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A, giving a high purification factor in a single step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose^™ 4 Fast Flow suitable for purification of monoclonal antibodies at both laboratory and process scale.

rProtein A Sepharose^™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, rProtein A Sepharose^™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

• High dynamic binding capacity due to oriented, single-point attachment of protein A
• Well-established Protein A medium used in many approved MAb processes
• No mammalian components involved during the manufacturing process

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

ligand: recombinant protein A (E. coli). shelf life: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.. packaging: pack of 5 . mL. manufacturer/tradename: Cytiva 17-1279-01. storage condition: (20% Ehtanol). matrix: 4% cross-linked agarose. particle size: 60-165 . μ. m. average diameter: 90 . μ. m (d50v). cleaning in place: 2-11. working range: 3-10. capacity: ~50 . mg binding capacity (human lgG/ml medium)(Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.). suitability: suitable for bioprocess medium. storage temp.: 2-8°C. Pictograms: GHS02. Signal Word: Warning. Hazard Statements: H226. Precautionary Statements: P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501. Storage Class Code: 3 - Flammable liquids.