RNASE A SOLUTION 1KIT WITH 1ML COMPONENT (C005B-232263)

General description

RNase A Solution is a convenient alternative to powdered RNase A. It is a highly purified preparation of bovine pancreatic ribonuclease A suitable for use in selective removal of RNA. It has been pretreated to remove DNase I and is suitable to remove RNA contamination during plasmid and genomic DNA purification procedures. Supplied at a concentration of 10 mg/ml in 10 mM Tris-HCl, 1 mM EDTA, 50% glycerol, pH 7.5.

Application

RNase A Solution has been used:

• in cell cycle profiling of embryonic mouse neuroectodermal cells (NE-4C)
• in the digestion of RNA for extraction of DNA from human osteosarcoma cell lines for chromatin immunoprecipitation (ChIP) assays
• to treat control adult and embryonic thymi cells during staining
• to remove RNA from affibody–mRNA fusion clones before clone screening

Biochem/physiol Actions

RNaseA is an endoribonuclease that catalyzes the degradation of phosphodiester linkages with a pyrimidine base at the 3′ -position on single-stranded RNA via, transphosphorylation and hydrolysis mechanism.

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

form: solution. manufacturer/tradename: Novagen^®. . storage condition: OK to freeze. concentration: 10 . mg/mL. technique(s): DNA purification: suitable. shipped in: ambient. storage temp.: 10-30°C. Pictograms: GHS08. Signal Word: Danger. Hazard Statements: H334. Precautionary Statements: P261 - P284 - P501. Hazard Classifications: Resp. Sens. 1. Storage Class Code: 10 - Combustible liquids. WGK: WGK 2.