Ribonuclease H (RNase H)

Nonspecific endoribonuclease that specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides.Source: E. coli H560 pol A1Storage Buffer: 25 mM Tris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 50% glycerol (v/v), pH 8.0 (+4°C)Volume Activity: 1 x 103 U/ml assayed according to Hillenbrand & Staudenbauer.Ribonuclease H (RNase H) is a nonspecific endoribonuclease, localized to the nucleus and cytoplasm. It is ubiquitously found and widely present among many organisms including viruses and human.Application:Ribonuclease H (RNase H) has been used for:In vivo RNA-primed initiation of DNA synthesisElimination of mRNA during second-strand cDNA synthesisSite-specific cleavage of RNADetection of RNA:DNA regions in double-stranded DNA of natural originRemoval of poly (A) sequences of mRNA if oligo (dT) is presentRNA extraction and quantitative reverse transcriptase polymerase chain reaction (RT-PCR)

Specification: from Escherichia coli H 560 pol A1