RESORUFIN

General description

Red fluorophore standard. Widely used in enzyme assay systems. Absorption and fluorescence of resorufin are pH dependent. Below the pKa (~6.0), Abs. shifts to ~ 480 nm and both E and fluorescence quantum yield are markedly lower. Resorufin is unstable in the presence of thiols such as dithiothreitol (DTT) and 2-mercaptoethanol.

Resorufin has high extinction coefficient and good chemical and photostability. These properties of resorufin make it perfect for its usage in inverse fluorescence assay to detect H₂O₂ by measuring the disappearance of its fluorescence upon horseradish peroxidase-mediated oxidation to a nonfluorescent derivative.

Molecular Weight: 213.19. Empirical Formula: C₁₂H₇NO₃. Quality Level: 100. Assay: ≥. 95.0% (HPLC), 95%. form: solid. mp: >. 300 . °C (lit.). storage temp.: −. 20°C. SMILES string: O: C(C: C1)C: C2C1: NC3: CC: C(O)C: C3O2. InChI: 1S/C12H7NO3/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9/h1-6,14H. InChI key: HSSLDCABUXLXKM-UHFFFAOYSA-N. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H315 - H319 - H335. Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338. Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3. Target Organs: Respiratory system. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.