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RENT TSS1.1RAT SPRAGUE-DAWLEY PRIMARY ENDOMETRIA (C005B-236897)

Catalog No.
C005B-236897
Manufacturer No.
92092515-1VL
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
VI

Cell Line Origin Rat Sprague-Dawley, primary endometrial, SV40 T antigen transformed Cell Line Description Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. The cells carry vimentin, CSF-1 (at 39°C),

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Cell Line Origin

Rat Sprague-Dawley, primary endometrial, SV40 T antigen transformed

Cell Line Description

Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. The cells carry vimentin, CSF-1 (at 39°C), desmin, collagen type III and connexin 43 markers. The transforming agent is a ts mutant of SV 40 T-antigen (tsA58).

Culture Medium

DMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Doubling time 48 hours. Cells show continuous growth at 34°C and growth arrest at 39°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source: rat endometrium. growth mode: Adherent. karyotype: Not specified. morphology: Cuboidal. products: Not specified. receptors: Glucocorticoid. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.
UPC:
42181505
Condition:
New
HazmatClass:
Yes
MPN:
92092515-1VL
Temperature Control Device:
Yes

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