RENCELL VM KIT

General description

ReNcell VM Kit contains human neural progenitor cells, optimized maintenance media and freezing media for culture of human neural stem cells. ReNcell VM Immortalized cells (SCC008): ReNcell VM is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. ReNcell VM was derived from the ventral mesencephalon region of human fetal brain. Immortalized by retroviral transduction with the v-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the ReNcell VM retains a normal diploid karyotype in culture even after prolonged passage (>45 passages). ReNcell VM was developed by the ReNeuron Group plc, a biotech company that specializes in using human somatic stem cells for therapeutics. In experiments performed by the ReNeuron Group plc, ReNcell VM can be differentiated in vitro to a high level of human dopaminergic neurons. Neurons differentiated from ReNcell VM have furthermore been shown to be electophysiologically active. ReNcell VM may be used for a variety of research applications such as studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions. ReNcell VM cells have been obtained in a legal and ethical manner, compliant with current local informed consent procedures. ReNcell NSC Maintenance Medium (SCM005): ReNcell Neural Stem Cell (NSC) Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of ReNcell immortalized human neural progenitor cells. When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of ReNcell immortalized VM and CX neural stem cells. Withdrawal of the growth factors from ReNcell NSC Maintenance Medium will result in the spontaneous differentiation of ReNcell immortalized neural progenitor cells. ReNcell NSC Freezing Medium (SCM007): ReNcell NSC Freezing Medium is qualified for use with ReNcell immortalized human neural progenitor cell lines, CX (CHEMICON Catalog No. SCC007) and VM (CHEMICON Catalog No. SCC008) cultured in serum-free conditions with ReNcell NSC Maintenance Medium (CHEMICON Catalog No. SCM005). The optimized formulation allows for consistent cryopreservation and high viability upon thawing and plating.

Cell Line Description

Neural Stem Cells

Application

Research Category
Stem Cell Research

Components

ReNcell VM Immortalized Cell LIne (Cat.# SCC008)

ReNcell NSC Maintenance Media (Cat.# SCM005)

ReNcell Neural Stem Cell Freezing Medium (Cat.# SCM007)

Quality

Each lot of ReNcell VM cells has been validated for high level of expression of Nestin and Sox 2 and for their self-renewal and multi-lineage differentiation capacities (please refer to datasheet figures). Cells also display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma. Maternal blood has been screened for HIV, HTLV, Hepatitis B and C.

Other Notes

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the "Academic Use Agreement" as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany