Recombinant GAPDH Antibody (C007B-026518)

Rabbit anti Human GAPDH Antibody, Recombinant (RR688), could be used for WB, IHC, Flow, IF, ICC, IP and so on.Application:WB: 1/5000 - 1/40000       IHC: 1/1600 - 1/3200Flow: 1/200 - 1/1000IF/ICC: 1/2000 - 1/10000IP: 1/50Protein FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Post-translationalS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.ISGylated.

Specifications and Purity: ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL