Rabbit anti-EBOV L pAb, 100ug

Immunogen: Peptide sequence specific to Zaire Ebola virus (ZEBOV) L-Polymerase protein. Description: Affinity purified rabbit polyclonal antibody reactive to Zaire Ebola virus L-Polymerase. The antibody detects LPolymerase by Western blot in ZEBOV infected HeLa cells. Supplied: 100 µg of affinity purified antibody is provided in PBS at a concentration of 0.93 mg/mL. 0.01% Sodium azide has been added. Raised in: Rabbits Purification: Antibody is affinity purified using immobilized immunogen. Clonality: Polyclonal Relevance: The antibody can be used for detection of ZEBOV LPolymerase Recommended Dilutions:  ELISA: Not Tested. WB: Reactivity to a ZEBOV (Mayinga) ebolavirus infected HeLa cells and uninfected HeLa cells was evaluated by Western Blot. Western blot detection was also performed to determine the cross-reactivity of the antibody to virus like particles (VLPs) of Zaire and Sudan ebola virus (ZEBOV and SEBOV) and Marburg virus (MARV) expressing glycoprotein, nucleoprotein and VP40. Storage: 2-3 weeks +4°C, -20°C long term.  Specificity: Antibody detects L-polymerase at the size of approximately 240 kDa in the HeLa infected lysate (Lane 2), but not the control uninfected lysate (Lanes 1). Cross Reactivity: Western Blot analysis shows that the antibody very weakly detects a band in 2 µg of MARV VLPs between 30-40 kDa but not ZEBOV and SEBOV VLPs. Also, in addition to detection of the expected L-polymerase, the anti-L antibody weakly detects two bands in the uninfected HeLa lysate between 35-52 kDa. The target of this reactivity is unknown. Reactivity of the antibody to L polymerase of other filovirus species is also unknown. Intended for research use only, not for human, therapeutic, or diagnostic applications.

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