Protein Purification from Tissue, For 6 groups of 4-5 or 24-30 students.

Proteomics often involves the purification and identification of novel proteins from tissues. The strategy often employed is one using multiple and different protein purification techniques to fractionate proteins into specific fractions. This allows for easier identification and characterization of novel proteins. The Purification of Proteins from Tissue kit is a comprehensive kit designed to teach students the principle of utilizing numerous purification techniques to identify novel proteins. Students will purify proteins from a mammalian tissue (mouse liver) and will use various fractionation and chromatography techniques to purify three target proteins from the mouse liver extract. The purification procedure will be closely monitored and easily visualized with gel electrophoresis. Students will then be able to assign specific characteristics to the isolated proteins, based on the purification techniques. This kit teaches protein fractionation, gel filtration and hydrophobic chromatography. Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher's Guide and separate Student's Guides. Applications Step-by-step approach to protein purification. Protein purification strategies. Perform salt fractionation, gel filtration and Hydrophobic chromatography.