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protein a-agarose (c15-1875-397)

PROTEIN A-AGAROSE (C005B-440024)

Catalog No.
C005B-440024
Manufacturer No.
P2545-5ML
Manufacturer Name
Sigma-Aldrich
Quantity
5
Unit of Measure
ML

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General description Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains. Application Protein A−:Agarose has been used in the immunoprecipitation of protein phosphatase and tensin homolog

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General description

Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.

Application

Protein A−Agarose has been used in the immunoprecipitation

  • of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells
  • of prolyl hydroxylase domain 2 N27 cells
  • of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells

Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Biochem/physiol Actions

Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG. Protein A agarose resin is commercially used for affinity based purifications.

Physical form

Suspension in 0.5 M NaCl containing preservative

UPC:
12352202
Condition:
New
HazmatClass:
No
MPN:
P2545-5ML

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