PROPIDIUM LODIDE/ ANTIFADE SOLUTION

General description

Propidium Iodide: {3,8-diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diodide}. Antifade Solution: p-Phenylenediamine and glycerol in PBS solution. 2.5μg/mL Propidium Iodide (PI) suspended in Antifade.

Application

Propidium Iodide is a nuclear fluorescent stain used for counterstaining paraffin-embedded, formalin-fixed tissue, to discriminate between live vs. dead cells in flow cytometry, and staining metaphase chromosomes or interphase nuclei that have been hybridized with probes detected with Fluorescein, or equivalent fluors. Antifade prevents the permanent loss of fluorescence or fluorescent signal quenching due to photobleaching and prolonged exposure to high intensity light sources.



Materials Provided

Each vial contains 1 ml of Propidium Iodide/Antifade Solution.



Warnings and Precautions

Propidium Iodide is a potential carcinogen. Antifade is a potential toxin and irritant. Use extreme caution. Avoid contact with eyes, skin and mucous membranes. Wear gloves and a lab coat when handling. Follow your institution′s guidelines for hazardous waste disposal. Keep tightly capped and limit exposure to light.



Related Products

Antifade Solution S7114

DAPI/Antifade Solution S7113

Propidium Iodide Solution S7109

Storage and Stability

Recommended Storage

-15ºC to -25ºC

Protect from light

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.