PMA FOR USE IN MOLECULAR BIOLOGY APPLICATIONS >=99% (HPLC) (C005B-440369)

General description

PMA (Phorbol 12-myristate 13-acetate) is an activator of protein kinase C (PKC) and a known tumor promoting agent. PMA also exhibits clastogenic (chromosome-breaking) activity.

Phorbol 12-myristate 13-acetate (PMA) can induce the activity of PLD1 (phospholipase D1) but not PLD2 (phospholipase D2).

Through the protein kinase C (PKC) signaling pathway, PMA is a tumor promoter associated with:

• gene transcription
• cell growth
• cell differentiation
• programmed cell death
• immune pathway
• receptor desensitization

• growth factors
• hormones
• cytokines-mediated regulation of cellular growth and differentiation

Application

PMA has been used:

• for the detection of intracellular cytokines
• in CellROX oxidative stress assay
• for Huh7 cell stimulation to investigate dipeptidyl peptidase 9 (DPP9) re-distribution
• as a positive control in B-cell receptor (BCR) stimulation of chronic lymphocytic leukaemia (CLL) cells
• in the differentiation of monocytes (THP -1) into macrophages

Suitable for

• molecular biology studies as standard
• analytical studies of tumor promoters
• promotion of cell growth

Biochem/physiol Actions

Potent tumor promoter; activates protein kinase C (PKC) in vivo and in vitro.

Potent tumor promoter; activates protein kinase C in vivo and in vitro.

Features and Benefits

• Stimulation of interleukin 2 production of Jurkat cells
• T-cell stimulation
• Protein kinase C (PKC) activator
• NF-κB Activator
• Promotion of cell growth
• Used in analytical studies of tumor promoters
• Molecular Biology grade

Caution

Photosensitive, protect from light.

Analysis Note

• Stimulation of interleukin 2 production of Jurkat cells
• T-cell stimulation
• Protein kinase C (PKC) activator
• NF-κB Activator
• Promotion of cell growth
• Used in analytical studies of tumor promoters
• Molecular Biology grade