PLASMINOGEN FROM BOVINE PLASMA (C005B-437941)

Biochem/physiol Actions

The activated form of plasminogen, plasmin, was shown to promote the migration of melanoma and lung tumor cells plated on the extracellular matrix protein laminin by altering protein kinase C signaling.

Plasminogen is the inactive precursor of the protease plasmin. Plasminogen is activated by the action of either tissue plasminogen activator (tPA), which primarily activates the fibrinolytic (thrombolytic) activity of plasmin, or urokinase plasminogen activator (uPA), which is associated with extracellular matrix remodeling and cell migration. Plasmin cleaves fibrin/fibrinogen and blood coagulation factors V/Va and VIII/VIIIa. It activates matrix metalloproteinases by cleaving the inactive proenzymes. It is also involved in the activation of some growth factors, such as vascular endothelial growth factor (VEGF) and transforming growth factor β (TGF-β).

Other Notes

ε-Aminocaproic acid free

Unit Definition

One unit will produce 1.0 μmole of p-nitroanilide from D-Val-Leu-Lys 4-nitroanilide at pH 7.5 at 37°C.

Physical form

Lyophilized powder containing NaCl, EDTA, lysine, and Tris buffer salts

Reconstitution

Solutions should be stored in buffer containing 20 mM lysine at neutral pH. The protein should be as concentrated as possible. Frozen in aliquots at −20 °C, it should be stable for several weeks.