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phosphatase inhibitor cocktail 1 (c15-1875-318)

PHOSPHATASE INHIBITOR COCKTAIL I (C005B-439959)

Catalog No.
C005B-439959
Manufacturer No.
P2850-1ML
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
ML

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General description Phosphatase Inhibitor Cocktail 1 includes a mixture of inhibitors that will inhibit the L -isozymes of alkaline phosphatase as well as serine/threonine protein phosphatases such as PP1 and PP2A. This product contains

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General description

Phosphatase Inhibitor Cocktail 1 includes a mixture of inhibitors that will inhibit the L-isozymes of alkaline phosphatase as well as serine/threonine protein phosphatases such as PP1 and PP2A. This product contains microcystinLR, cantharidin, and (−)-p-bromotetramisole. Phosphatase Inhibitor Cocktail 1 has been replaced with P0044.

Application

Phosphatase Inhibitor Cocktail 1 has been used in nuclear protein isolation and electrophoretic mobility shift assay (EMSA) for catenin activation. It has also been used in western blot analysis.

Biochem/physiol Actions

Phosphatases are active regulators of a number of important biological processes. Inhibition of phosphatases by small molecules is useful to study its biological role.

Quantity

Recommended usage is a 100-fold dilution.

Analysis Note

Tested on cell extracts from animal tissues such as bovine liver, human placenta, and rabbit muscle, as well as on extracts from A431 and Jurkat cell lines.

UPC:
51191904
Condition:
New
HazmatClass:
Yes
MPN:
P2850-1ML
Temperature Control Device:
Yes

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