PHENOL FOR MOLECULAR BIOLOGY STAB. (C005B-007181)

General description

Phenol/carbolic acid is mainly produced by hospital pharmacy as it is unavailable in premixed liquid form. It is not highly soluble in water. Phenol should be refrigerated and protected from light exposure to maintain its shelf life of approximately one year. Kidneys can excrete phenol as several conjugated derivatives.

Application

Phenol has been used in the incubation of pellet for RNA extraction.

Biochem/physiol Actions

Phenol has the ability to denature protein, hence can lead to denervation. At lower concentration, it can serve as a local anaesthetic and can also act as a neurolytic agent in higher concentration. It is also linked with tissue damage at higher concentrations.

Other Notes

For the preparation of DNA ; Large- and small-scale phenol extractions

Molecular Weight: 94.11. Empirical Formula: C₆H₆O. linearFormula: C₆H₅OH. grade: for molecular biology. Quality Level: 100. vapor density: 3.24 (vs air). vapor pressure: 0.09 psi ( 55 °C), 0.36 mmHg ( 20 °C). product line: BioUltra. Assay: ≥. 99.5% (GC). form: solid. autoignition temp.: 1319 . °F. contains: ~0.15% hypophosphorous acid as stabilizer. expl. lim.: 8.6 . %. impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤. 0.5% water. ign. residue: ≤. 0.05%. pH: 3.0-6.0 (25 . °C, 0.5 . M in H₂O). bp: 182 . °C (lit.). mp: 40-42 . °C (lit.), 40-42 . °C. solubility: H₂O: 0.5 . M at . 20 . °C, clear, colorless. density: 1.071 . g/mL . at 25 . °C (lit.). anion traces: chloride (Cl^-): ≤. 5 . mg/kg, sulfate (SO₄^2-): ≤. 50 . mg/kg. cation traces: Al: ≤. 5 . mg/kg, As: ≤. 0.1 . mg/kg, Ba: ≤. 5 . mg/kg, Bi: ≤. 5 . mg/kg, Ca: ≤. 10 . mg/kg, Cd: ≤. 5 . mg/kg, Co: ≤. 5 . mg/kg, Cr: ≤. 5 . mg/kg, Cu: ≤. 5 . mg/kg, Fe: ≤. 5 . mg/kg, K: ≤. 50 . mg/kg, Li: ≤. 5 . mg/kg, Mg: ≤. 5 . mg/kg, Mn: ≤. 5 . mg/kg, Mo: ≤. 5 . mg/kg, Na: ≤. 50 . mg/kg, Ni: ≤. 5 . mg/kg, Pb: ≤. 5 . mg/kg, Sr: ≤. 5 . mg/kg, Zn: ≤. 5 . mg/kg. SMILES string: Oc1ccccc1. absorption: cut-off at 294 . nm in H₂O at 0.5 . M. suitability: suitable for molecular biology. storage temp.: 2-8°C. InChI: 1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H. InChI key: ISWSIDIOOBJBQZ-UHFFFAOYSA-N. Pictograms: GHS05,GHS06,GHS08,GHS09. Signal Word: Danger. Hazard Statements: H301 + H311 + H331 - H314 - H341 - H373 - H411. Precautionary Statements: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2. Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials. WGK: WGK 2. Flash Point(F): 174.2 °F - closed cup. Flash Point(C): 79.0 °C - closed cup. Personal Protective Equipment: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges.