Pfu DNA Polymerase, 5u/uL, 500u

Pfu DNA Polymerase, 5u/ul: Pfu DNA Polymerase, 5u/ul Product Description: Cloned Pfu DNA polymerase is isolated from the hyperthermophilic marine archaebacterium. Pyrococcus furiosus. The multinational thermostable enzyme possesses both 5'- to 3'- DNA polymerase and 3'- to 5'- exonuclease activity, which results in a 12-fold increase in fidelity of DNA synthesis over Pfu DNA polymerase. Cloned Pfu DNA Polymerase has a temperature optimum between 72°C and 78°C remains > 95% active following one hour incubation at 95°C. 10 x Reaction Buffer: 200 mM TrisHCl ( pH 8.8) 100 mM KCl 100 mM (NH4)2 SO4 20 mM MgSO4 1% Triton X-100 1 mg / ml nuclease-free bovine serum albumin (BSA) Unit Activity: One unit of activity is the amount of cloned Pfu DNA polymerase required to incorporate 10nM of [H] TTP into an acid-insoluble form in 30 minutes at 72°C. Contaminants: Cloned Pfu DNA polymerase is tested extensively for the absence of detectable nonspecific nuclease activity and DNA contamination.