Peptidoglycan from Bacillus subtilis

DescriptionPeptidoglycan.is a biopolymer that is a major component of the cell walls of Gram-positive bacteria. Peptidoglycan consists of a carbohydrate backbone of alternating units ofN-acetyl glucosamine andN-acetyl muramic acid and is modified with both D- and L-amino acids.N-Acetyl muramic acid residues are cross-linked with oligopeptides. The terminal peptide is D-alanine although other amino acids are present as D-isomers.Peptidoglycan may be used for the activity estimation of lytic enzymes (e.g. lyticase). It is recommended to use a peptidoglycan concentration of 0.15-3 μg/ml in water or buffer and measuring at 450 nm. The peptidoglycan is not soluble but produces a suspension in water.Peptidoglycan activates the Toll-like receptor 2 (TLR2), present in mammalian cells for the stimulation of lymphocytes. It may also function as an antagonist of Poly (I:C).

Specifications and Purity: ≤1.00EU/mg Endotoxin

MDL Number: MFCD00212486