OMNIPUR(R) CHAPS 1PC X 100GM (C005B-077244)

General description

Zwitterionic detergent ideal for solubilization of membrane proteins, analytical, diagnostic, chromatography, electrophoresis work, and enzymology and immunology applications.
Suitable for Molecular Biology

Conductivity (10%, water): ≤50 µmho
DNase: None detected
Protease: None detected
Purity (TLC): Single spot
RNase: None detected
Solubility: 20% in water, passes test





Intended for laboratory and manufacturing use only. Not for drug, food, or household use.

Application

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.
Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

OMNIPUR is a registered trademark of Merck KGaA, Darmstadt, Germany

Molecular Weight: 614.88. Empirical Formula: C₃₂H₅₈N₂O₇S. Quality Level: 100. form: crystalline powder. mol wt: micellar avg mol wt 6150. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze. aggregation number: 10. color: white. CMC: 6 . mM (20-25°C). transition temp: cloud point >. 100 . °C. foreign activity: DNAse, none detected, Protease, none detected, RNAse, none detected. shipped in: ambient. storage temp.: 10-30°C. SMILES string: C[C@H](CCC(: O)NCCC[N+](C)(C)CCCS([O-])(: O): O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C. InChI: 1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1. InChI key: UMCMPZBLKLEWAF-RFCNGIAKSA-N. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H302. Precautionary Statements: P264 - P270 - P301 + P312 - P501. Hazard Classifications: Acute Tox. 4 Oral. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.