Product DescriptionChromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity on native (ds) DNA undetectable under the assay conditions. A frozen solution in 30mM sodium acetate, pH 4.6, 50mM NaCl, 1mM ZnCl2, and 50% glycerol.Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn2+ and/or Ca2+. Inhibitors are EDTA, citrate and high concentrations of SDS.Product ApplicationNuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA.It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor.
Specifications and Purity: ≥100,000-500,000 units/ml
MDL Number: MFCD00131010
PubChem CID: 135298688
Related Documents: https://aladdin-for-icloud-store.oss-cn-hangzhou.aliyuncs.com/aladdinsci/pdp/sds/1/N128634-SCI_2740f8c3ecc3e5300bd4d4fd632158fb.pdf
- UPC:
- 41106104
- Condition:
- New
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- N128634-10KU
- CAS:
- 37288-25-8
- Product Size:
- 10KU
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