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nthy-ts1human thyroid follicular mutantepitheli (c15-1311-532)

NTHY-TS1HUMAN THYROID FOLLICULAR MUTANTEPITHELI (C005B-235531)

Catalog No.
C005B-235531
Manufacturer No.
90011610-1VL
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
VI

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Cell Line Origin Human thyroid follicular mutant epithelial Cell Line Description Normal primary cultures of human thyroid follicular cells were transfected with a plasmid containing the SV40 early region large T encoding gene from mutant tsA58.

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Cell Line Origin

Human thyroid follicular mutant epithelial

Cell Line Description

Normal primary cultures of human thyroid follicular cells were transfected with a plasmid containing the SV40 early region large T encoding gene from mutant tsA58. Tight temperature dependence for growth is observed: Nthy-ts1 cells grow at 33°C, but not at the restrictive temperature of 40.5°C. This SV40 mutant allows the study of oncogene action free of any interfering effects of SV40 large T antigen.

Application

Transfection, mutation and virus studies: SV40

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 33°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source: human thyroid. growth mode: Adherent. karyotype: Not specified. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.
UPC:
51181608
Condition:
New
HazmatClass:
Yes
MPN:
90011610-1VL
Temperature Control Device:
Yes

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