NORMAL RABBIT SERUM

General description

Normal carrier sera from healthy, non-immunized rabbits.

Application

ELISA (see comments)

Flow Cytometry (see comments)

Immunoblotting (see comments)

Immunofluorescence (see comments)

Immunohistochemistry (see comments)

Immunoprecipitation (see comments)

Warning

Toxicity: Standard Handling (A)

Physical form

Serum diluted in 250 mM NaCl, 10 mM PBS, pH 7.6.

Reconstitution

Reconstitute the lypohilized diluted serum in 1 ml sterile distilled water with occasional gentle mixing for at least 2 h at 4°C. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.

Other Notes

Normal sera are provided to be used as blocking agents in immunohistochemical procedures or as negative controls for undiluted serum/rabbit polyclonal antibodies from the Calbiochem brand. For use as a negative control, perform experiments in parallel using primary antibody and normal serum at the same concentrations. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction. This antibody has also been reported to work for ELISA, flow cytometry, immunoblotting, immunofluorescence, immunohistochemistry, and immunoprecipitation.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany