NORMAL MOUSE IGG (C005B-477954)

General description

Native IgG from mouse serum. IgG is a glycoprotein found in serum and tissue fluids. For use as a negative control in parallel with specific mouse IgG-type primary antibodies.

Application

ELISA (see comments)

Flow Cytometry (see comments)

Immunoblotting (see comments)

Immunofluorescence (see comments)

Immunohistochemistry (see comments)

Immunoprecipitation (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Other Notes

Normal IgG are provided to be used as negative controls for purified antibodies from Calbiochem. For use as a negative control, perform experiments in parallel using primary antibody or normal serum at the same concentration. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany