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nitric oxide assay kit

NITRIC OXIDE ASSAY KIT

Catalog No.
C005B-447992
Manufacturer No.
MAK454-1KT
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
KT

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General description Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase, is involved in host defense and development, activation of

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General description

Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase, is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules.

Features and Benefits

  • Sensitive and accurate. Detection range 0.6 - 200 μM in 96-well plate.
  • Compatible with high-throughput handling systems.

Suitability

The kit is suitable for the quantitative determination of nitric oxide (nitrate/nitrite) and evaluation of drug effects on its metabolism in plasma, serum, urine, tissue, cells and foods.

Principle

Simple, direct and automation-ready procedures for measuring NO are becoming popular in research and drug discovery. Since NO is oxidized to nitrite and nitrate, it is common practice to quantitate total NO2-/NO3- as a measure for NO level. The Nitric Oxide Assay Kit is designed to accurately measure NO production following reduction of nitrate to nitrite using an improved Griess method. The procedure is simple and the time required for sample pretreatment and assay is reduced to as short as 30 minutes.

UPC:
41116007
Condition:
New
HazmatClass:
Yes
MPN:
MAK454-1KT
Temperature Control Device:
Yes

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