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ninhydrin reagent according to stahl

NINHYDRIN REAGENT ACCORDING TO STAHL

Catalog No.
C005B-097360
Manufacturer No.
17975-100ML
Manufacturer Name
Sigma-Aldrich
Quantity
100
Unit of Measure
ML

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Ninhydrin reagent is mostly used for the detection of amino acids. It basically gives a characteristic purple color on reaction with a-amino acids and other amino compounds, provided the pH of the reaction is 5.

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General description

Ninhydrin reagent is mostly used for the detection of amino acids. It basically gives a characteristic purple color on reaction with a-amino acids and other amino compounds, provided the pH of the reaction is 5.0 or higher. At an acid pH of 0.3, the reaction of ninhydrin with ornithinine and proline results in a red color product. Ninhydrin reagent can stay stable at room temperature in the absence of nitrogen atmosphere for upto 1 month in the ethylene glycol-sodium acetate solvent system.

Application

1 g ninhydrin dissolved in 475 mL 1-butanol and 25 mL acetic acid

Ninhydrin reagent may be used for the quantitative determination of total protein content in tissue hydrolysates.

grade: for TLC derivatization. Quality Level: 100. form: liquid. technique(s): thin layer chromatography (TLC): suitable. impurities: 1-butanol, acetic acid. density: 0.823 . g/mL . at 20 . °C. Pictograms: GHS02,GHS05,GHS07. Signal Word: Danger. Hazard Statements: H226 - H302 - H315 - H318 - H335 - H336. Precautionary Statements: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2. Storage Class Code: 3 - Flammable liquids. WGK: WGK 2. Flash Point(F): 93.2 °F - closed cup. Flash Point(C): 34 °C - closed cup. Personal Protective Equipment: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter.
UPC:
51131509
Condition:
New
HazmatClass:
Yes
MPN:
17975-100ML

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