G-Trap Ni IDA Agarose Fast Flow, 5 x 5mL column

G-TrapTM NiIDA Agarose Fast Flow columns are ready to use prepacked affinity columns used for purification of proteins based on immobilized metal ion affinity chromatography (IMAC). IMAC is based on interaction of cations of transition metals such as Ni2+, Co2+ with protein residues histidine, cysteine and to some extent to tryptophan. In G-TrapTM Ni IDA Agarose Fast Flow , Ni 2+ are immobilized on cross-linked 6% agarose using iminodiacetic acid groups (IDA) which are covalently bound to resin. G-TrapTM Ni IDA Agarose Fast Floware specifically designed for purification of recombinant proteins fused with 6x histidine (6XHis) tag. Other G-TrapTM IDA Agarose Fast Flow column available in 1 ml and 5 ml column volume is G-TrapTM CoIDA Agarose Fast Flow . One can chose between appropriate column as per requirement. The properties of G-TrapTM Ni IDA Agarose Fast Flow resin and the Column are listed below. G-TrapTM Ni IDA Agarose Fast Flowresin Matrix : Cross-linked agarose beads, 6% Mean particle size : 90 μm Chelating Agent : Iminodiacetic acid Active group : Ni 2+ Metal iondensity : 20-40 μmol /ml Binding Capacity: 5-10mg His-tagged protein/ml medium pH stability Working Range : 3-12 pH stability Cleaning-in-Place (CIP): 2-14 Maximum Flow Velocity : 2-14 Recommended Flow rate : 1 ml/min and 5 ml/min for 1 ml and 5 ml column respectively Exclusion limit(globular proteins) : 4 x 106 Chemical Stability : Stable to all commonly used aqueous buffers: 8 M urea, 6 M guanidine hydrochloride Storage Conditions : 2 to 8°C, 20% Ethanol G-TrapTM Ni IDA Agarose Fast Flow?, 1 ml columns Column Volume : 1 ml Columns Dimension : 0.7 x 2.5 cm Column Harware Pressure Limit: 0.5 MPa Column Hardware : Polypropylene (Biocompatible) G-TrapTM Ni IDA Agarose Fast Flow, 5 ml columns Column Volume : 5 ml Columns Dimension : 1.6 x 2.5 cm Column Harware Pressure Limit: 0.5 MPa Column Hardware : Polypropylene (Biocompatible) Application(s) : G-TrapTM Ni IDA Agarose Fast Flow columns are ready to use prepacked affinity columns used for purification of proteins based on immobilized metal ion affinity chromatography (IMAC).G-TrapTM Ni IDA Agarose Fast Flow are specifically designed for purification of recombinant proteins fused with 6x histidine (6XHis) tag.