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nek2, active human

NEK2 ACTIVE HUMAN RECOMBINANT EXPRESSED IN ESCHERICHIA COLI PROTEIN APPARENT MOL WT ~80 KDA AQUEOUS GLYCEROL SOLUTION

Catalog No.
C005B-444217
Manufacturer No.
N4787-5UG
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
EA

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Application Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 °C using a final concentration of 50 μ:M [ 32 P] ATP. Biochem/physiol Actions Nek2

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Application

Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.

Biochem/physiol Actions

Nek2 (nimA-related kinase 2) is a cell cycle-regulated protein kinase with maximal activity at the onset of mitosis that localizes to the centrosome.

Unit Definition

One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP

Physical form

Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.

UPC:
12141728
Condition:
New
HazmatClass:
No
MPN:
N4787-5UG
Temperature Control Device:
Yes

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