NAD APPROX. 100% GRADE I LYO. 5G

General description

Nicotinamide adenine dinucleotide (NAD) is a ubiquitously found electron carrier and a cofactor. NAD⁺ contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD⁺ complexes, the enzyme-cofactor interactions are highly conserved.

Application

NAD (nicotinamide adenine dinucleotide), Grade I has been used in the kinetic studies of human class IV alcohol dehydrogenase.

NAD has been used in in vitro glycogen synthase kinase 3 (GSK3) deacetylation assay. It has also been used as a component in assay mix for the determination of kinetic characteristics using an enzyme-linked kinetic assay.

Biochem/physiol Actions

NAD is also involved in microbial catabolism. β-NAD acts as a substrate for various enzymes in several cellular processes.

The NAD (nicotinamide adenine dinucleotide)/NADH ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo. It works as an electron acceptor.

Electron acceptor

Packaging

Packaged by solid weight.

Quality

Purity: 100% β-NAD based on dry weight (enzymatically, CN-complex and absorbance); A₂₅₀nm - A₂₆₀nm : 0.83 ± 0.02, A₂₈₀nm - A₂₆₀nm : 0.22 ± 0.02
Absence of contaminants: LDH inhibitors are not detectable
Organic solvents: =0.05% (GC)

Specifications

Absorbance coefficients: 17.6 x 10³l x mol^-1 x cm^-1 at 260nm, 5.9 x 10³l x mol^-1 x cm^-1 at 333nm (CN complex)

Preparation Note

Storage conditions (working solution): 10 mg/ml in aqueous solution, contains at least 99% NAD after 4 weeks at 2 to 8 °C

Other Notes

For life science research only. Not for use in diagnostic procedures.

This is the common form of NAD.

Molecular Weight: 663.43 (anhydrous basis). Empirical Formula: C₂₁H₂₇N₇O₁₄P₂ · xH₂O. Assay: 100% (dry weight). form: lyophilized. mol wt: M_r 663.4. packaging: pkg of 1 . g (10127965001), pkg of 5 . g (10127973001). manufacturer/tradename: Roche. color: off-white. pH: ~3.0 (50 . mg/mL in water). λ. _max: 260 nm. shipped in: wet ice. storage temp.: 2-8°C. SMILES string: NC1: NC: NC2: C1N: CN2.O[C@@H]3[C@@H](COP(O)(OP(OC[C@@H](O4)[C@@H](O)[C@@H](O)[C@H]4[N+]5: CC: CC(C(N): O): C5)([O-]): O): O)OC[C@@H]3O. InChI: 1S/C21H27N7O14P2/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33/h1-4,7-8,10-11,13-16,20-21,29-32H,5-6H2,(H5-,22,23,24,25,33,34,35,36,37)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1. InChI key: BAWFJGJZGIEFAR-NNYOXOHSSA-N. Storage Class Code: 13 - Non Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.