N-HYDROXYSUCCINIMIDE-ACTIVATED SEPHAROSE 4 FAST FLOW (C005B-200764)

General description

N-Hydroxysuccinimidyl-Sepharose^™ 4 Fast Flow is a cross-linked and preactivated matrix obtained by mixing Sepharose 4 Fast Flow with 6-aminohexanoic acid through a spacer arm. Esterification with N-hydroxysuccinimide activates the terminal carboxyl group. Ligand-containing primary amino groups directly interact with this active ester to form a chemically very stable amide linkage.

Application

N-Hydroxysuccinimidyl-Sepharose^™ 4 Fast Flow has been used as a part of a magnetic particles-based visual detection system to achieve separation upon binding of the amplified gene. It has also been used to immobilize protein during pull-down assay.

Features and Benefits

• Specifically recommended for coupling of small amino-containing proteins and peptides in process-scale applications
• High level of pre-activation, which gives a high degree of substitution of the selected ligand
• NHS coupling method enables chemically stable ligand attachment

Legal Information

Sepharose is a trademark of Cytiva

form: isopropanol suspension. Quality Level: 200. extent of labeling: 16-23 . μ. mol per mL. matrix: fast-flow highly cross-linked 4% beaded agarose. matrix active group: N-hydroxysuccinimide active ester. matrix spacer: 6 atoms (when ligands are coupled by displacement of NHS). storage temp.: 2-8°C. Pictograms: GHS02,GHS07. Signal Word: Danger. Hazard Statements: H225 - H319 - H336. Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338. Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3. Target Organs: Respiratory system. Storage Class Code: 3 - Flammable liquids. WGK: WGK 1. Flash Point(F): 53.6 °F. Flash Point(C): 12 °C. Personal Protective Equipment: Eyeshields, Gloves, type ABEK (EN14387) respirator filter.