MSVIF-TK+MOUSE BALB/C EMBRYONIC FIBROBLAST M-MS (C005B-235612)

Cell Line Origin

Mouse BALB/c embryonic fibroblast, M-MSV transformed

Cell Line Description

Derived from the MSV.B line (Sigma Catalogue number. 90031901) which has been exposed to 50 IU/ml Interferon for over 200 passages. Unlike the parent line, the cells are non-malignant and do not form tumours in nude mice.

Application

Interferon, transformation and virus studies: M-MSV

Culture Medium

EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) or RPMI 1640 + 2mM Glutamine + 50-100 IU/ml murine IFN + 5-10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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biological source: mouse embryo. growth mode: Adherent. karyotype: Not specified. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.