MODIFIER DIGOXIGENIN-NHS ESTER

General description

Chemical reagent for the labeling of proteins and oligonucleotides. One reactive group is able to interact with NH₂-groups (of proteins or oligonucleotides), another reactive group (Digoxigenin) reacts with Anti-Digoxigenin and thus can be detected.

Application

Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester has been used to label the sequence (III) for the linker molecule sequence (VIII).

Biochem/physiol Actions

Digoxigenin, a non-radioactive immune-tag is mainly used for labeling proteins and in situ hybridization.

Principle

The activated ester reacts with amino groups under mild conditions. The digoxigenin moiety can be introduced into proteins or 5′-amino substituted oligonucleotides.

Preparation Note

Working concentration: 1 to 5 mM
Working solution: Preparation of working solution

Under stirring 0.327 mg digoxigenin-3-O-methylcarbonyl- ε-aminocaproic acid -N-hydroxysuccinimide ester is dissolved in 8.18 μl DMSO or ethanol.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Molecular Weight: 658.78. Empirical Formula: C₃₅H₅₀N₂O₁₀. Quality Level: 100. Assay: >. 90% (elementary analysis). form: powder. mol wt: M_r 658.8. packaging: pkg of 5 . mg. manufacturer/tradename: Roche. pH range: 2-13. solubility: DMF: soluble, DMSO: soluble, ethanol: soluble. storage temp.: −. 20°C. SMILES string: [H][C@]12CC[C@]3([H])[C@]([H])(C[C@@H](O)[C@]4(C)[C@H](CC[C@]34O)C5: CC(: O)OC5)[C@@]1(C)CC[C@@H](C2)OCC(: O)NCCCCCC(: O)ON6C(: O)CCC6: O. InChI: 1S/C35H50N2O10/c1-33-13-11-23(45-20-28(39)36-15-5-3-4-6-31(42)47-37-29(40)9-10-30(37)41)17-22(33)7-8-25-26(33)18-27(38)34(2)24(12-14-35(25,34)44)21-16-32(43)46-19-21/h16,22-27,38,44H,3-15,17-20H2,1-2H3,(H,36,39)/t22-,23+,24-,25-,26+,27-,33+,34+,35+/m1/s1. InChI key: KHNDABJZSPPYLE-FUGFVFQCSA-N. Pictograms: GHS06,GHS08,GHS09. Signal Word: Danger. Hazard Statements: H300 - H319 - H331 - H373 - H410. Precautionary Statements: P260 - P264 - P273 - P301 + P310 + P330 - P391 - P403 + P233. Hazard Classifications: Acute Tox. 2 Oral - Acute Tox. 3 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - STOT RE 2. Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.