FLOW CYTOMETRY KIT FOR APOPTOSIS (C005B-257466)

General description

Metabolic Syndrome has received increased attention from the medical community with increasing cases of obesity, type 2 diabetes and related cardiovascular diseases being described and studied.

MILLIPLEX^® Human Apolipoprotein Panel is a 6-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, tissue culture supernatant or other biological samples: APOA-1, APOA-2, APOB, APOC-2, APOC-3, and APOE. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cardiovascular

Specificity

Cross Reactivty
To test cross-reactivity among the assays in the panel, individual apolipoprotein standard was prepared at a concentration 4 times greater than the highest concentration in the calibration curve and tested in the 6-plex assays with multiplexed beads containing immobilized capture antibodies and individual detection antibodies. There was negligible cross-reactivity within the panel.

Application

• Analytes: Apo AI, Apo AII, Apo B, Apo CII, Apo CIII, Apo E
• Recommended Sample Type: Human serum, plasma, tissue culture supernatant or other biological samples
• Recommended Sample Dilution: 10 μL of a 1: 4,000 dilution of plasma or serum
• Assay Run Time: Primary incubation of 1 hour followed by a secondary incubation of 30 minutes, both at room temperature (20-25°C)
• Research Subcategory: Cardiovascular Disease

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.

Standard curve range: Refer to the kit protocol for details.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.