Menadione (NSC 4170)

InformationMenadione (NSC 4170) Menadione (NSC 4170, Vitamin K3), a fat-soluble compound, is an inhibitor of Cdc25 phosphatase and mitochondrial DNA polymerase γ (pol γ) , used as a nutritional supplement.In vitroMenadione prevents mitochondrial Ca(2+) uptake in normal pancreatic acinar cells, which permits rapid spread of Ca(2+) throughout the cell. Menadione-induced mitochondrial depolarisation is due to induction of the permeability transition pore. Menadione (nontoxic concentration) results in a brief activation of extracellular signal-regulated/xa0kinase (ERK) and c-Jun N-terminal kinase (JNK) in the RALA255-10G rat/xa0hepatocyte/xa0cell line. Menadione is equipotent against multidrug-resistant and parental leukemia/xa0cell/xa0lines/xa0with IC50 values of 13.5 mM and 18 mM, respectively. Menadione/xa0inhibits the incorporation of radioactive thymidine, uridine and amino acids into DNA, RNA and protein, respectively, in three/xa0humancancer cell lines. Menadione/xa0at 1-20 mM dose- and time-dependently inhibits cell proliferation of/xa0AR4-2J/xa0cells./xa0Menadione/xa0(100 mM) causes rapid cell death. Menadione/xa0(100 mM) induces DNA smear in electrophoresis indicative of/xa0necrosis, while lower concentrations (10-20 mM) induces a DNA ladder indicative of/xa0apoptosis. Menadione/xa0at 1-20 mM induces wild-type P53, whereas the 100 mM/xa0menadione/xa0has a minor effect on wild-type P53. Menadione/xa0treatment clearly affects the mitochondrial function of Jurkat T cells by inducing a collapse of the inner transmembrane potential (DeltaPsi(m)) and a decrease in inner membrane mass, which could be completely reversed by N-acetylcysteine.In vivoCell Datacell lines：Concentrations：Incubation Time：Powder Purity:≥99%.

Specifications and Purity: 10mM in DMSO

Molecular Formula: C11H8O2

Molecular Weight: 172.18

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