MAGNA CHIP HT96 CHROMATIN IMMUNOPRECIPITATION KIT

General description

The Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format. This reliable streamlined approach works for both cells and tissues and can be performed using either a multi-channel pipette or an automated liquid handling instrument.Each kit includes a complete set of validated, quality controlled reagents, plus a detailed protocol.

Features & Benefits

• Complete set of materials for up to 96 ChIP reactions
• Protein A+G bead blend for ChIP with a broader range of antibodies than A or G alone
• Low Chromatin requirements: 10, 000 to 100, 000 cells per reaction
• Optimized streamlined protocol with only a single buffer for sonication, IP, or wash; and protocols for automated liquid handling systems
• Protocols for using cells or tissues
• Direct analysis of DNA without additional clean-up steps
• Compatible with ChIPAb+ validated antibody and primer sets

Application

Research Category
Epigenetics & Nuclear Function

Used to detect/quantify: Protein A+G

Packaging

Kit capacity: 96 chromatin immunoprecipitation assays

Components

Magna ChIP protein A/G magnetic beads

HT96 Nuclei Isolation Buffer

HT96 ChIP Buffer (Sonication/ChIP/Wash)

Low Stringency IP Wash Buffer

HT96 ChIP Elution Buffer

Proteinase K Solution

Protease Inhibitor Cocktail III

10X Glycine

10X PBS

96 Well ChIP Plate

96 Well Thermal Plate

Plate Seal

Strip Caps

Physical form

Two boxes containing all necessary reagents to perform 96 chromatin immunoprecipitation reactions in a 96 well plate format. Supplied buffers are sufficient to generate 24 individual chromatin preparation reactions using 15 cm plates of cultured cells or tissue samples.

Storage and Stability

Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level: 100. manufacturer/tradename: Magna ChIP^®. . technique(s): immunoprecipitation (IP): suitable. shipped in: dry ice. Pictograms: GHS07. Signal Word: Warning. Precautionary Statements: P305 + P351 + P338. Storage Class Code: 10-13 - German Storage Class 10 to 13.