LYSOZYME ACTIVITY KIT

General description

The Lysozyme Detection Kit provides ready-to-use reagents for detecting the presence of lysozyme activity. This simple assay to detect lysozyme activity uses Micrococcus lysodeikticus cells as the substrate. Lysozyme activity results in the lysis of the Micrococcus lysodeikticus cells. During incubation of the lysozyme sample and substrate, the reaction is followed by monitoring the decrease in absorbance at 450 nm.

Application

Lysozyme Activity Kit has been used to measure the catalytic activity of native lysozyme, lysozyme purified from chicken egg white and lysozyme from pilocarpine-stimulated saliva.

Biochem/physiol Actions

Lysozyme hydrolytically cleaves the bonds between N-acetyl-D-glucosamine and N-acetylmuramic acid residues (GlcNAc β1-4Mur) in mucopolysaccharides and the glycan skeletons of the corresponding peptidoglycans (the latter are glycosaminoglycans linked to or crosslinked by peptide chains). Lysozyme effectively destroys the cell walls of bacteria.

Storage and Stability

The Reaction Buffer is stable for at least 2 years at 2–8 °C.
The Lysozyme (Catalog Number L6876) may be stored long term at –20 °C and remains active for 4 years.
The Micrococcus lysodeikticus cells (Catalog Number M3770) should be stored long term at –20 °C.